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SARS-CoV-2 Rapid SmartAmp Assay Kit

Rapid, accurate and highly specific Isothermal DNA Amplification.

Diagnostic samples

In partnership with EMG

Evotech Mirai Genomics is a joint Japanese-Russian company that develops proprietary products and technology for rapid diagnostics of Covid 19.

The MSE Group is the distribution partner of EMG in Europe.

State-of-the-art technology

Fast

Less than 40 minutes

Sensitivity

100 copies / reaction

Specificity

100% demonstrated

Reverse transcription using AMV Reverse Transcriptase

Isothermal DNA amplification with SmartAmp® Technology using Aac DNA Polymerase

  • Unique and highly stable target region within ORF1 (Nsp15)
  • Longer primers for higher specificity
  • Asymmetric primer design to favour self priming elongation and minimize mispriming events

Real time monitoring of amplification with Eprobe® Technology

  • Sequence specific monitoring for higher specificity

Easy protocol

Components:

Tube 1 : Reagent P SmartAmp® primers for initiation of amplification and Eprobe® primers for real-time monitoring of amplification

Tube 2 : Reagent E Enzymes for reverse transcription and DNA amplification

Tube 3 : Positive Control Synthetic RNA fragments of SARS-CoV-2

Tube 4 : Negative Control Nuclease free distilled water

Expiry Date: 12 months from production date. Do not use an expired Kit.

Infinite Compatibility

Works on real time PCR instruments using the following settings:

  • Temperature : +67°C
  • Cycles: 40 x 1 minute
  • Detection channel: FAM
  • Detection : every minute (at the end of each cycle)

Examples of compatible real time thermal cyclers:

  • Roche LightCycler 96
  • Roche LightCycler ® 480 I/II (instructions available)
  • Thermo Fisher Scientific Quantstudio ™ 5 (instructions available)
  • Qiagen Rotor Gene Q (instructions available)
  • Bio-Rad CFX96

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